Role of Endophyte Chaetomium Globosum Lk4 in Growth of Capsicum Annuum by Producion of Gibberellins and Indole Acetic Acid
نویسندگان
چکیده
Endophytic fungi represent a trove of unexplored natural source of plant hormones like gibberellins (GAs) and indoleacetic acid (IAA). In present study, we isolated eight endophytes from the roots of drought stressed pepper (Capsicum annuum L.) plants. To assess phytohormones secreting potential, culture filtrates (CF) of endophytes were screened on GAs biosynthesis mutant Waito-C rice. Endophyte CAC-1G significantly promoted the shoot growth, chlorophyll content and biomass of Waito-C rice seedlings as compared with CF of Fusarium fujikuroi and distilled water. CAC-1G was identified as strain of Chaetomium globosum LK4 by sequencing internal transcribed spacer regions and phylogenetic analysis of similar sequences. The CF analysis of C. globosum showed the presence of GAs (GA1 0.67±0.13 ng/ml; GA4 21.8±1.2 ng/ml; GA9 0.51±0.11 ng/ml; GA1213.4±0.41 ng/ml; GA20 1.11±0.2 ng/ml) and IAA (16.71+1.42 μg/ml). The CF of C. globosum had higher GA4, GA12 and GA20 than the CF of F. fujikuroi. The CF containing propagules of C. globosum was applied to the host-pepper plants. The results revealed significantly higher shoot growth, chlorophyll content, plant biomass and leaf area as compared to fungal-free medium and water applied plants. The present results of C. globosum can be reciprocated for improved plant growth and yield at field levels.
منابع مشابه
بررسی اثرات آللوپاتیک عصاره آبی کنگر فرنگی فعالیت آنزیم های آنتی اکسیدان، فعالیت آنزیم آلفا آمیلاز و غلظت درونی هورمون های گیاهی ریزوم اویار سلام ارغوانی در مرحله جوانه زنی
In order to evaluate the allelopatic potential of artichoke (Cynara cardunculus) on germination, growth, lipid peroxidation and some hormones content of Cyperus rotundus rhizome were assessed in Islamic Azad University, Shoushtar Branch (Iran). The experiments were carried out under completely randomized design with 6 treatments (0, 20%, 40%, 60%, 80% and 100% C. cardunculus aqueous extract as ...
متن کاملAntimicrobial activity of Curcuma longa L., Capsicum annuum L. and Piper nigrum at different conditions
Background: In addition to creating the desired flavor, spices play an important role in increasing the shelf life of food by inhibiting the growth of a variety of microorganisms. Objective: The purpose of this study was to investigate the antibacterial effect of boiled and non-boiled water extract of Curcuma longa L., Capsicum annuum L. and Piper nigrum alone or in combination. Methods: The an...
متن کاملEndophyte Chaetomium globosum D 38 and its 1 elicitors promote tanshinones accumulation of Salvia 2 miltiorrhiza 3
متن کامل
Bioproduction of Indole Acetic Acid by Rhizobium Strains Isolated from Root Nodules of Green Manure Crop, Sesbania sesban (L.) Merr.
Twenty six Rhizobium strains were isolated from root nodules of Sesbania sesban (L.) Merr. collected from different regions of Andhra Pradesh. All the 26 Rhizobium strains produced indole acetic acid (IAA), but maximum amount was produced by only five strains in yeast extract mannitol (YEM) medium supplemented with L-tryptophan. The strains were found to elaborate maximum IAA when fed with 2.5...
متن کاملSeed germination-influencing bioactive secondary metabolites secreted by the endophyte Cladosporium cladosporioides LWL5.
The present study was aimed to isolate bioactive metabolites produced by a fungal endophyte from Helianthus annuus, Capsicum annuum, and Cucumis sativus and to assess their role in seed germination. Culture filtrate of the endophyte HA-3B from H. annuus was significantly inhibitory towards the germination and growth of lettuce seeds. HA-3B was identified as Cladosporium cladosporioides LWL5 thr...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
دوره شماره
صفحات -
تاریخ انتشار 2012